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style="font-size:12pt;">PCR</span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒</span></span></span></strong> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;"></span></span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">产品目录号</span></span></span></strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:</span></span></span><span style="color:black;"><span style="font-size:12pt;">NHS21-5</span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">高</span></span></span></strong><strong><span style="color:black;"><span style="font-size:12pt;">GC</span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">纳米</span></span></span></strong><strong><span style="color:black;"><span style="font-size:12pt;">PCR</span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒产品说明</span></span></span></strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:本试剂盒既适用于高</span></span></span><span style="color:black;"><span style="font-size:12pt;">GC</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">序列的扩增,也适用于一般</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">扩增。产品定位于解决目前市场上难于扩增的若干</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">反应。</span></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">纳米</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">是纳米材料辅助的基因扩增技术</span></span></span><span style="color:black;"><span style="font-size:12pt;">,</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">研究表明某些纳米材料可以增强基因扩增的总体效率。</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">高</span></span></span></strong><strong><span style="color:black;"><span style="font-size:12pt;">GC</span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">纳米</span></span></span></strong><strong><span style="color:black;"><span style="font-size:12pt;">PCR</span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒</span></span></span></strong><span style="color:black;"><span style="font-size:12pt;">25ul/250</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">次</span></span></span>  <span> </span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">配置如下:</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">2</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR buffer 11-1 (1000ul)</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">;</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">2</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR buffer 11-2 (1000ul)</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">;</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">2</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR buffer 11-3 (1000ul)</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">;</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">dNTPs (2.5mM)(300ul)</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">;</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">MgCl<sub>2</sub><span> </span>(25 mM) (500ul)</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">;</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">Taq DNA polymerase (5 U/ul) (60ul)</span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">使用建议</span></span></span></strong><strong><span style="color:black;"><span style="font-size:12pt;">:</span></span></strong> </p><ul style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"><li> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">纳米材料容易聚集,每次使用前请震荡均匀再使用。</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">产物中含有的纳米材料一般不影响电泳等后续操作。</span></span></span> </li> <li> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">一个典型的</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">反应(举例:扩增一个高</span></span></span><span style="color:black;"><span style="font-size:12pt;">GC</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">片段</span></span></span><span style="color:black;"><span style="font-size:12pt;">780bp</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">)设计如下:</span></span></span> </li></ul><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;">  </p><table align="center" style="color:#555555;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;border-collapse:collapse;background-color:#FFFFFF;" border="1" cellspacing="0" cellpadding="0"><tbody><tr><td style="vertical-align:top;"> <p>   </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">12ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">反应</span></span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">25ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">反应</span></span></span><span style="color:black;"><span style="font-size:12pt;"></span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">2</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR buffer-11</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">6.0 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">12.0ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">dNTPs (2.5 mM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.0 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">2.0ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">MgCl<sub>2</sub><span> </span>(25 mM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.2 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">2.5ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Primer 1(2 uM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.8 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.6ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Primer 2(2 uM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.8 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.6ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Template DNA (~10 ng/ul)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.2 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.4ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Taq DNA polymerase (5 U/ul)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.2 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.4ul</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">ddH<sub>2</sub>O</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.8 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">4.5ul</span></span> </p> </td> </tr></tbody></table><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">程序举例:</span></span></span><span style="color:black;"><span style="font-size:12pt;">[94</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">℃</span></span></span><span style="color:black;"><span style="font-size:12pt;">-5min]</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">;</span></span></span><span style="color:black;"><span style="font-size:12pt;">[94</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">℃</span></span></span><span style="color:black;"><span style="font-size:12pt;">-30s</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">,</span></span></span><span style="color:black;"><span style="font-size:12pt;">56</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">℃</span></span></span><span style="color:black;"><span style="font-size:12pt;">-30s</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">,</span></span></span><span style="color:black;"><span style="font-size:12pt;">72</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">℃</span></span></span><span style="color:black;"><span style="font-size:12pt;">-40s]</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">35 cycles</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">(循环数可按照</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">产物的多少适当调节);</span></span></span><span style="color:black;"><span style="font-size:12pt;">[72</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">℃</span></span></span><span style="color:black;"><span style="font-size:12pt;">-5min].<span> </span></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">如果不确定zui佳退火温度,可以先做梯度</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">确定zui佳退火温度。</span></span></span> </p><ul style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"><li> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">批量</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">需要计算并规划好实验中所需要混合的</span></span></span><span style="color:black;"><span style="font-size:12pt;">mix</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">总量以及需要单独加入</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">体系中的成分和加量。例:做以不同基因组为模板的</span></span></span><span style="color:black;"><span style="font-size:12pt;">9</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">管</span></span></span><span style="color:black;"><span style="font-size:12pt;">12ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">体系的</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">。计入加样损耗,按</span></span></span><span style="color:black;"><span style="font-size:12pt;">10</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">管加量计算。试验操作全过程建议在冰盒上进行。</span></span></span> </li></ul><table align="center" style="color:#555555;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;border-collapse:collapse;background-color:#FFFFFF;" border="1" cellspacing="0" cellpadding="0"><tbody><tr><td style="vertical-align:top;"> <p>   </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">12ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">反应</span></span></span> </p> </td> <td style="vertical-align:top;"> <p>   </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">2</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR buffer-11</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">6.0 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=60</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">dNTPs (2.5 mM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.0 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=6</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">MgCl2 (25 mM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.2 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=12</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Primer 1(2 uM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.8 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=8</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Primer 2(2 uM)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.8 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=8</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Template DNA (~10 ng/ul)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.2 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">单独添加</span></span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">Taq DNA polymerase (5 U/ul)</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">0.2 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=2</span></span> </p> </td> </tr><tr><td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">ddH2O</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-size:12pt;">1.8 ul</span></span> </p> </td> <td style="vertical-align:top;"> <p> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">×</span></span></span><span style="color:black;"><span style="font-size:12pt;">10=18</span></span> </p> </td> </tr></tbody></table><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;">  </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">按照上表所描述的将</span></span></span><span style="color:black;"><span style="font-size:12pt;">mix</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">做好,并反复摇晃</span></span></span><span style="color:black;"><span style="font-size:12pt;">15-20</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">下、上下振荡后离心(大约待离心机转到</span></span></span><span style="color:black;"><span style="font-size:12pt;">7000rpm</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">随即停下来就好),离心后,用枪头分别加入相应基因组</span></span></span><span style="color:black;"><span style="font-size:12pt;">0.2ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">的</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">管内,每管</span></span></span><span style="color:black;"><span style="font-size:12pt;">11.8ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">并用枪头顺便混合</span></span></span><span style="color:black;"><span style="font-size:12pt;">3</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">下使均匀。放入</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">仪进行</span></span></span><span style="color:black;"><span style="font-size:12pt;">PCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">。</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;">  </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">实验举例</span></span></span></strong><strong><span style="color:black;"><span style="font-size:12pt;">1</span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:</span></span></span></strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">扩增以人类基因组为模板的</span></span></span><span style="color:black;"><span style="font-size:12pt;">38</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">对序列引物。该</span></span></span><span style="color:black;"><span style="font-size:12pt;">38</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">对高</span></span></span><span style="color:black;"><span style="font-size:12pt;">GC</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">扩增产物</span></span></span><span style="color:black;"><span style="font-size:12pt;">GC&gt;60%,</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">大小均在</span></span></span><span style="color:black;"><span style="font-size:12pt;">700-800bp</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">左右。</span></span></span><span style="color:black;"><span style="font-size:12pt;">38</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">对退火温度基本一致。使用上述</span></span></span><span style="color:black;"><span style="font-size:12pt;">12ul</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">扩增体系,</span></span></span><span> </span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">使用</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒进行扩增的图片如图</span></span></span><span style="color:black;"><span style="font-size:12pt;">1</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">所示。</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;"><img style="width:564px;height:112px;" alt="说明: 高GC-38自己的产品图片可以用" src="https://web.archive.org/web/20220113043714im_/file://c/Users/li/AppData/Local/Temp/msohtmlclip1/01/clip_image002.jpg"></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">图</span></span></span><span style="color:black;"><span style="font-size:12pt;">1<span> </span></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">使用</span></span></span><span style="color:black;"><span style="font-size:12pt;">NanoPCR</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒扩增人类基因组</span></span></span><span style="color:black;"><span style="font-size:12pt;">38</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">对引物的电泳图片</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">Marker</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">为λ</span></span></span><span style="color:black;"><span style="font-size:12pt;">/Hind<span> </span></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">Ⅲ</span></span></span><span style="color:black;"><span style="font-size:12pt;">.</span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;">  </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-size:12pt;"><img style="width:564px;height:95px;" alt="说明: 高GC-1" src="https://web.archive.org/web/20220113043714im_/file://c/Users/li/AppData/Local/Temp/msohtmlclip1/01/clip_image004.jpg"></span></span></strong> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">图</span></span></span><span style="color:black;"><span style="font-size:12pt;">2<span> </span></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">使用国外某公司高</span></span></span><span style="color:black;"><span style="font-size:12pt;">GC</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒扩增人类基因组</span></span></span><span style="color:black;"><span style="font-size:12pt;">38</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">对引物的电泳图片</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;">  </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;"><img style="width:564px;height:107px;" alt="说明: 高GC-2" src="https://web.archive.org/web/20220113043714im_/file://c/Users/li/AppData/Local/Temp/msohtmlclip1/01/clip_image006.jpg"></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">图</span></span></span><span style="color:black;"><span style="font-size:12pt;">3 <span> </span></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">使用国内某公司高</span></span></span><span style="color:black;"><span style="font-size:12pt;">GC</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">试剂盒扩增人类基因组</span></span></span><span style="color:black;"><span style="font-size:12pt;">38</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">对引物的电泳图片</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;">  </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">参考文献</span></span></span></strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">Haikuo Li etal.  Nanoparticle PCR: Nanogold-assisted PCR with enhanced specificity. Angew. Chem. Int. Ed. 2005, 44(32):5100-3</span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">Zhizhou Zhang et al. Aqueous suspension of carbon nanopowder enhances the efficiency of polymerase chain reaction. Nanotechnology, 18 (2007) 355706</span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;">Zhizhou Zhang et al. Aqueous suspension of carbon nanotubes enhances the efficiency of long PCR. 2008, Biotechniques, 44(4):537-545</span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">保存温度</span></span></span></strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:</span></span></span><span style="color:black;"><span style="font-size:12pt;">-20</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">℃</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">保质期</span></span></span></strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:</span></span></span><span style="color:black;"><span style="font-size:12pt;">12</span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">个月</span></span></span> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">咨询</span></span></span></strong><strong><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">:</span></span></span></strong> </p><p style="color:#555555;text-indent:0px;font-family:Tahoma;font-size:14px;font-style:normal;font-weight:400;background-color:#FFFFFF;"> <span style="color:black;"><span style="font-size:12pt;"></span></span><span style="color:black;"><span style="font-family:宋体;"><span style="font-size:12pt;">QQ:</span></span></span><span 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