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② 手工操作中,加样板过多造成加样后放入孵箱前等待时间过长(特别是室内温度较高时); ③ 加完标本再加酶试剂时酶溅出孔外。解决办法: ① 标本为血清:最好将血液先自然存放1-2小时后,再用3000rmp离心15分钟;标本为血浆:必须使用含抗凝剂的血液标本收集管,采血后必须立即颠倒采血管混合5-10次,放置一段时间后,3000rpm离心15分钟;若在几天内检测,可放在2-8℃冰箱中,若要贮存,则置于-20℃的低温冰箱内。 ② 加样后及时放入孵箱。 ③ 加酶试剂后用吸水纸在酶标板表面轻拭吸干。④ 如果采用AT或其他全自动加样,最好选择FAME或其他后处理仪器加酶试剂。 ⑤ 标本较多时,请分批操作。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 3. 孵育</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 可能原因: ① 孵育时未贴封片或加盖,使标本或稀释液蒸发,吸附于孔壁,难于清洗彻底; ② 孵育时间人为延长,导致非特异性结合紧附于反应孔周围,难以清洗彻底。解决办法:① 贴封片或加盖; ② 按说明步骤严格控制操作时间。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 4. 洗板</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 可能原因: ①采用手工洗板,孔与孔之间液体交叉。 ② 采用半自动洗板机洗板时,洗液量不足,导致洗板不彻底;洗板针堵塞,抽吸不完全;洗板不畅,导致洗板效果差。 ③ 反应板过多造成洗板等待时间长。 解决办法: ① 保证洗液注满各孔,洗板针畅通,洗完板后最好在吸水纸(选择干净、无或少尘的吸水材料)上轻轻拍干; ② 合理安排,或多用几台洗板机。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 5. 显色</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 可能原因: ① 显色剂配制后放置时间过长或使用过期显色剂; ②加显色剂时溅出孔外造成液体回流。解决办法: ① 显色剂尽量在临用前配制,坚持不用过期显色剂,肉眼可见浅蓝色的TMB显色剂不用; ② 加样时保持显色剂不外流; ③ A、B液应避免接触金属器械。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 6. 终止</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 可能原因如加终止液时产生较多气泡,导致假阳性增加。所以在加终止液时应避免产生气泡。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 7. 读板</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 如读板时板底不清洁等。应保证酶标板清洁。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 所以在整个操作过程中保证酶标板不接触次氯酸;尽可能实现ELISA检测标准自动化,有效提高检测质量。</div><div style="margin:0px auto;padding:0px;color:#666666;font-family:Arial, 宋体B8B体;"> 在实际操作中,除了选择优良试剂外,必须严格按照操作步骤进行操作,同时作好室内质控、室间质评,以严谨的工作作风检测每一份标本,才能保证检测质量。现在国内已有相当数量的单位拥有全自动酶标仪,这对于实现ELISA标准化检测、提高检测质量起到了重要作用。</div></li> <li class="n_back"><a href="javascript:history.go(-1);">返回列表</a> </li> </ul></div> </div> </div> </div> </div> </div> <div id="foot"> <div class="foot"> <div class="wrap"> <div class="foot_left"> <div class="foot_b"> <div class="foot_p"> 产品&amp;服务</div> <ul class="foot_ul"><li><a href="/web/20180826010156/http://husbio.com/products/23.aspx">ELISA试剂盒</a></li> <li><a href="/web/20180826010156/http://husbio.com/products/24.aspx">抗体抗原</a></li> <li><a href="/web/20180826010156/http://husbio.com/products/25.aspx">生化试剂</a></li> <li><a href="/web/20180826010156/http://husbio.com/products/26.aspx">标准品/对照品</a></li> </ul></div> <div class="foot_b"> <div class="foot_p"> 新闻媒体</div> <ul class="foot_ul"><li><a href="/web/20180826010156/http://husbio.com/news/30.aspx">行业动态</a></li> <li><a href="/web/20180826010156/http://husbio.com/news/29.aspx">公司新闻</a></li> </ul></div> <ul class="foot_ul1"><li><a href="/web/20180826010156/http://husbio.com/about.aspx">关于户实</a></li> <li><a href="/web/20180826010156/http://husbio.com/products.aspx">产品&amp;服务</a></li> <li><a href="/web/20180826010156/http://husbio.com/kycx.aspx">科研创新</a></li> <li><a href="/web/20180826010156/http://husbio.com/news.aspx">新闻媒体</a></li> <li><a href="/web/20180826010156/http://husbio.com/partners.aspx">合作伙伴</a></li> <li><a href="/web/20180826010156/http://husbio.com/contact.aspx">联系我们</a></li> </ul></div> <div class="foot_right"> <div class="foot_logo"> <img src="/web/20180826010156im_/http://husbio.com/templates/demo/images/foot_logo.jpg"></div> <div class="foot_dianhua"> 联系电话:<span>18916004157</span></div> </div> </div> </div> <div class="foot_txt"> <div class="wrap"> 上海户实医药科技有限公司版权所有 沪ICP备15045113号-1 <a target="_blank" href="https://web.archive.org/web/20180826010156/http://www.beian.gov.cn/portal/registerSystemInfo?recordcode=31011502005588" style="display:inline-block;text-decoration:none;height:20px;line-height:20px;"> <img src="/web/20180826010156im_/http://husbio.com/news_show/templates/demo/images/bottmo_1.png" style="float:left;"><span style="color:#ffffff;">沪公网安备 31011502005588号</span> </a></div> </div></div> <style>*{margin:0;padding:0;list-style-type:none;}a,img{border:0;}/* side */.side{position:fixed;width:54px;height:221px;right:0;top:214px;z-index:100;}.side ul li{width:54px;height:54px;float:left;position:relative;border-bottom:1px solid #ddd; 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